Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi dan Didiamkan 30 menit Sebelum Disentrifugasi

Silvia, Silvia (2022) Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi dan Didiamkan 30 menit Sebelum Disentrifugasi. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background : Triglyceride examination was carried out in three stages, namely pre-analytical, analytical, and post-analytical stages. The pre-analytical stage of specimen processing is one of the factors that can affect triglyceride levels. High levels of triglycerides can cause atherosclerosis to happen or cause coronary heart disease. In order to get the serum, it is done by allowing the blood to clot by itself for ± 30 minutes and then the blood is centrifuged at 3000 rpm for 5 - 15 minutes. However, in the field are often that the processing for triglyceride test specimen examination oftenis not in accordance with applicable regulations. Therefore, this study aims to see the differences of triglyceride levels in blood sample was immediately centrifuged and left for 30 minutes before being centrifuged. Objective : Knowing the examination results of triglyceride levels examined in the blood and was immediately centrifuged and left for 30 minutes before being centrifuged. Method : This research is a pre-experimental research design with intact Group Comparison. The research subjects used were 30 TLM students. Blood samples were collected in 2 clot activator tubes, then the first tube was immediately centrifuged and the second tube was left for 30 minutes before being centrifuged. Results : The results showed that the average triglyceride level in the blood that immediately centrifuged was heigher than the blood that was left for 30 minutes before being centrifuged. There for, It is stated that there is a significant difference in the p value = 0.001 (p<0.05). Conclusion : Based on the research, it was concluded that there was a significant difference in the examination of triglyceride levels. Between blood sample that was immediately centrifuged and the blood that was left for 30 minutes before being centrifuged.

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