Perbedaan Kadar Asam Urat Pada Serum Hemolisis (1g/L) dan Tidak Hemolisis Menggunakan Metode Uricase

Sitinjak, Yeni Debora (2022) Perbedaan Kadar Asam Urat Pada Serum Hemolisis (1g/L) dan Tidak Hemolisis Menggunakan Metode Uricase. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: uric acid levels checking in the main specimen, is namely serum. Serum is obtained from blood processing by centrifugation. Improper use of taking blood specimens using 3cc can cause in vitro hemolysis which can affect the results of the examination. This hemolysis occurs at the pre-analytic stage in time taking the specimen, the specimen is still used for examination. Blood sprayed spontaneously to the botol of the tube without releasing the needle, causing the erythrocytes to burst, that affect free hemoglobin in the serum. Objective: To know the differences between uric acid levels in hemolyzed serum (1g/L) and non-hemolytic serum using the uricase method Methods : the method of this research is a pre-experimental research with One Group Pretest-Posttest research design. The subjects in this study were 36 people who met the inclusion and exclusion criteria. Examination using the uricase method. Using the kenza 450 TX tool. Statistical test using Paired T-test with 95% confidence level. Results: The results showed an increase in the average level of uric acid in hemolyzed serum of 1 g/L and non-hemolytic. Conclusion : There is a significant differences between uric acid levels in hemolyzed serum (1g/L) and non-hemolytic serum using the uricase method with a Sig 2 tailed value <0.05. Suggestion: To confirm the diagnosis, the sample used in the examination of uric acid is non-hemolytic serum and for the next researchers, a similar study can be carried out, by comparing the condition of serum in (ureum, creatinine) with lower hemolysis levels such as 0.5 g/L and 0 ,25 g/L

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