Pengaruh Variasi Waktu Aklimatisasi Media Mueller Hinton Terhadap Diameter Zona Hambat Bakteri Escherichia coli Menggunakan Antibiotik Tetrasiklin

Patricia, Yona Putri (2026) Pengaruh Variasi Waktu Aklimatisasi Media Mueller Hinton Terhadap Diameter Zona Hambat Bakteri Escherichia coli Menggunakan Antibiotik Tetrasiklin. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Antibiotic susceptibility testing is an important laboratory examination used to determine bacterial susceptibility to antibiotics. The accuracy of the test results is influenced by several factors, including the pH of the medium, agar thickness, inoculum density, temperature, and incubation time. One technical factor that is often overlooked is the acclimatization process of Mueller Hinton agar, which is the adjustment of the medium temperature from refrigerated storage to room temperature before use. This study used Escherichia coli and tetracycline antibiotic discs. Objective: To determine whether variations in the acclimatization time of Mueller Hinton agar affect the inhibition zone diameter of Escherichia coli using tetracycline antibiotic discs. Methods: This study employed a pre-experimental design with a static group comparison approach. The samples used were Escherichia coli and tetracycline antibiotic discs. Each treatment was repeated ten times based on the Federer formula. Data were analyzed using SPSS version 22 through descriptive analysis, normality testing, one-way ANOVA, and Tukey's post hoc test. Results: The results of the one-way ANOVA and Tukey's post hoc test showed no significant effect of variations in the acclimatization time of Mueller Hinton agar on the inhibition zone diameter of Escherichia coli, with a significance value of 0.080 (p > 0.05). Conclusion: There was no significant effect of variations in the acclimatization time of Mueller Hinton agar on the inhibition zone diameter of Escherichia coli using tetracycline antibiotic discs (p > 0.05). Suggestion: Future researchers are recommended to compare acclimatization times using different bacterial species.

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