Optimasi Variasi Waktu Perendaman Kertas Saring Menggunakan Ceftriaxone Injeksi pada Diameter Zona Hambat Bakteri Escherichia coli

Hia, Oktafianus (2026) Optimasi Variasi Waktu Perendaman Kertas Saring Menggunakan Ceftriaxone Injeksi pada Diameter Zona Hambat Bakteri Escherichia coli. Undergraduate thesis, Universitas Katolik Musi Charitas Palembang.

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Abstract

Background: Antibiotic susceptibility testing is a method used to determine the sensitivity of bacteria to antibiotics. Escherichia coli is a bacterium commonly encountered in microbiological examinations and has the potential to cause infections. Ceftriaxone is an effective antibiotic for the treatment of Escherichia coli infections. Ceftriaxone discs used for susceptibility testing are relatively expensive and have limited availability. Therefore, filter paper impregnated with ceftriaxone injection solution can be used as an alternative to commercial antibiotic discs. Ceftriaxone injection is more cost-effective and can serve as an alternative to commercial ceftriaxone discs. Objective: Determining the optimal soaking time of filter paper in ceftriaxone injection solution on the inhibition zone diameter of Escherichia coli. Methods: This study was a pre-experimental study with a static group comparison design using Escherichia coli as the test bacterium. Each treatment was performed in 10 replications based on Federer’s formula. The data were analyzed using the Kruskal-Wallis test followed by Dunn’s post hoc test. Results: The mean inhibition zone diameter of Escherichia coli using a ceftriaxone disk was 31 mm, whereas filter paper immersed in ceftriaxone injection solution for 20, 25, and 30 minutes produced mean inhibition zone diameters of 25.1 mm, 25.6 mm, and 26 mm, respectively. The Kruskal–Wallis test showed a significant difference among the groups (p < 0.000), and the Dunn post hoc test indicated that all immersion groups differed significantly from the standard disk group (p < 0.05). Conclusion: The optimal filter paper immersion time in ceftriaxone injection solution was 30 minutes, resulting in a mean Escherichia coli inhibition zone diameter of 26 mm. Suggestion: Future studies are recommended to evaluate soaking times of up to 1 hour and to assess the potential use of Whatman No. 3 filter paper as an alternative to commercial antibiotic discs.

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