Perbedaan Jumlah Koloni Candida Albicans Pada Media Emb (Eosin Methylen Blue) Dan Sda (Sabouroud Dextrose Agar)

Yudistara, Regen (2019) Perbedaan Jumlah Koloni Candida Albicans Pada Media Emb (Eosin Methylen Blue) Dan Sda (Sabouroud Dextrose Agar). Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background : Identification of C. albicans fungus in the laboratory can be done by culturing the fungus C. albicans in the media SDA (Sabouroud Dextrose Agar) which is a selective media and standard medium for culturing the fungus C. albicans but not all labs have SDA media, for that is what it takes media alternatives to growing the fungus C. albicans one media EMB (eosin Methylen Blue) containing carbohydrates and protein that is comparable with SDA media. This study was conducted to determine the amount of colonies of Candida albicans Differences In Media EMB (eosin methylene blue) and SDA (Sabouroud Dextrose Agar). Method : This type of research is the study of True experiment with posttest study design Only Control Group Design. This study uses the media EMB (eosin methylene blue) and SDA (Sabouroud Dextrose Agar) with each - each group numbered 16. The fungus C. albicans were inoculated on EMB media and SDA medium and incubated at 37 , Then the data were analyzed usingPaired sample t-test with a 95% confidence level. Results: At SDA media C. albicans fungal growth occurs with the average - average 153.75 colonies CFU / mL and EMB media C. albicans fungal growth occurs with the average - average 189.38 colonies CFU / mL. The test results showed statistically significant differences (0,007) in SDA media and EMB. Conclusion: Pursuant to the results of the study there are differences in the growth of the number of C. albicans fungal colonies growing on SDA media and EMB media.

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