Perbedaan Jumlah Koloni Jamur Tricophyton Mentagropytes Pada Media Sabauroud Dextrose Agar (Sda) Dan Media Sabauroud Dextroseagar (Sda) Dengan Penambahan Glukosa 3 Gram

Mardiyana, Mega (2019) Perbedaan Jumlah Koloni Jamur Tricophyton Mentagropytes Pada Media Sabauroud Dextrose Agar (Sda) Dan Media Sabauroud Dextroseagar (Sda) Dengan Penambahan Glukosa 3 Gram. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: The most common culture medium used to regrow and reproduce a genus of fungi isSabauroudDextrose Agar (SDA) with a specific composition set. The present study aims to see the effect of glucose as the additional substrate in SDA culture medium to boostTrichophyton mentagrophytes.To be more specific, the main purpose is to investigate the effect of providing addition of 3 grams glucose on SDA medium to enhance the colonies numbers of Trichophyton mentagrophytes. Method:The study is experimental research with research design of posttest-only control design. The sample used in this research is Trichophyton mentagrophyteswhich is a genus of fungi. The number of fungi is standardized using Mac Farland standard and diluted with more diluent until 102. The data are obtained by calculating the colonies number of Trichophyton mentagrophytes.on SDA medium and the mixture of SDA medium and 3 grams glucose. Result:There are 32 samples which have been inoculated and calculated to get the colonies number of Trichophyton mentagrophytes. To be more specific, there is 16 sample of Trichophyton mentagrophyteswhich are regrown on SDA culture medium, while the rest 16 samples are regrown on the mixture medium of SDA and 3 grams of glucose. Paired t-test confirmed that the result of sig 2-tailed indicates 0,967 which is higher than the significance level (α = 0,05) (p> 0,05). Conclusion: The result shows that there is no significant difference on colonies number between the culture grown on SDA and the mixture medium of SDA and 3 grams of glucose, indicating the additional glucose does not provide any effect on colonies number.

Item Type: Thesis (Undergraduate)
Additional Information: Skripsi lengkap dapat dibaca di Ruang Referensi Perpustakaan UKMC Kampus Burlian
Uncontrolled Keywords: Keywords: Glucose, SabauroudDextrose Agar,Trichopyton mentagrophytes
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Divisions: Undergraduate Theses - D3/D4 > Medical Laboratory Technology Diploma Study Program
Depositing User: Perpustakaan Unika Musi Charitas
Date Deposited: 13 May 2020 05:33
Last Modified: 13 May 2020 05:33
URI: http://eprints.ukmc.ac.id/id/eprint/3625

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