Perbedaan Zona Inhibisi Uji Kepekaan Antibiotik Golongan Aminoglikosida (Gentamisin Dan Amikasin) Terhadap Bakteri Staphylococcus Aureus Pada Suhu Inkubasi 37℃

Febrina, Kurnia (2019) Perbedaan Zona Inhibisi Uji Kepekaan Antibiotik Golongan Aminoglikosida (Gentamisin Dan Amikasin) Terhadap Bakteri Staphylococcus Aureus Pada Suhu Inkubasi 37℃. Undergraduate thesis, Universitas Katolik Musi Charitas.

	Text TLM-2019-1534035-cover.pdf Download (179kB)
	Text TLM-2019-1534035-abstract.pdf Download (17kB)
	Text TLM-2019-1534035-tableofcontent.pdf Download (24kB)
	Text TLM-2019-1534035-chapter1.pdf Download (84kB)
	Text TLM-2019-1534035-chapter2.pdf Download (166kB)
	Text TLM-2019-1534035-chapter2.pdf Restricted to Repository staff only Download (166kB)
	Text TLM-2019-1534035-chapter3.pdf Restricted to Repository staff only Download (124kB)
	Text TLM-2019-1534035-chapter4.pdf Restricted to Repository staff only Download (141kB)
	Text TLM-2019-1534035-conclusion.pdf Download (24kB)
	Text TLM-2019-1534035-reference.pdf Download (53kB)
	Text TLM-2019-1534035-attachment.pdf Restricted to Repository staff only Download (915kB)
	Text TLM-2019-1534035-complete.pdf Restricted to Repository staff only Download (1MB)

Abstract

Background: Staphylococcus aureus is a type of bacterium which can promote disease by making an invasion of human tissue. According to World Health Organisation (WHO), the optimum temperature to regrow the bacteria is 35ºC. The higher temperature will increase the bacterium sensitivity. However, the sensitivity test is considered as an important way to see the sensitivity of Staphylococcus aureus bacterium against several antibiotics. Disc diffusion is one of the most common sensitivity tests of bacterium or microorganism. Currently, this study is conducted to investigate the different inhibition zone of Staphylococcus aureus bacterium using gentamicin and amikacin as the antibiotics models. The study is conducted at the incubation temperature of 37ºC which is higher than the optimum temperature condition to regrow the bacteria based on WHO recommendation. Method: This present study is analytical observation with a comparative cross-sectional study. The study is conducted at Microbiology Laboratory of Faculty of Medical Science, Universita Katolik Musi Charitas. The sample used in this study is Staphylococcus aureus which has been inoculated on Mueller Hinton Agar medium. To see the sensitivity of the bacterium, the small amount of gentamicin and amikacin antibiotics is added to the culture medium using Kirby-Bauer method. The medium is then incubated at 37ºC for 24 hours. After 24 hours, the inhibition zone is measured in millimeter unit, and the obtained data is then statistically analyzed using Mann-Whitney test with a confidence level of 95%. Result: From the result of the inhibition zone of gantamicin and amicacin antibiotic class aminoglycoside sensitivity tests showed the median inhibition zone obtained from the experiment is 23 mm with the minimum and maximum inhibition zone of 20 mm and 25 mm. Furthermore, statistical analysis found that there is no significant difference in the antibiotic inhibition zone of gentamicin and amikacin. It is based on the significant value which shows sig = 0,803. Conclusion: there is no significant difference in the antibiotic inhibition zone of gentamicin and amikacin using Staphylococcus aureus bacterium which incubated at 37ºC.

Actions (login required)

View Item