Perbandingan Jumlah Koloni Bakteri Klebsiella Pneumoniae Pada Media Eosin Methylene Blue (Emb) Dan Media Endo Agar Plate (Eap)

Putri, Dwi Annisa (2019) Perbandingan Jumlah Koloni Bakteri Klebsiella Pneumoniae Pada Media Eosin Methylene Blue (Emb) Dan Media Endo Agar Plate (Eap). Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Klebsiella pneumoniae is a normal flora bacteria which commonly found on tractus digestivus. The laboratory measurement can be conducted by calculating the number of germ or bacteria which is inoculated on Eosin Methylene Blue and Endo Agar Plate culture medium. The present study aims to compare the number of bacterial colonies of Klebsiella pneumoniae which is regrown on Eosin Methylene Blue Agar and Endo Agar Plate culture medium. Method: This present study is true experiment research which uses Klebsiella pneumoniae as the main sample. The study began by regrowing Klebsiella pneumoniae on Eosin Methylene Blue and Endo Agar Plate. All the deposited- Klebsiella pneumoniae is incubated at the temperature of 37oC for 48 hours and calculated. Furthermore, the experiment is repeated for 16 times to prove all the obtained data repeatable. All the obtained data is statistically analyzed using paired T-test with a confidence level of 95%. Result: The result shows that the average number of Klebsiella pneumoniae bacteria colonies grown on Eosin Methylene Blueand Endo Agar Plate are 67 colonies forming unit (CFU)/ml and 64 colonies forming unit (CFU)/ml, respectively. Furthermore, statistical analysis using paired T-test confirmed that there is no significant difference of Klebsiella pneumoniae colonies number grown on Eosin Methylene Blue and Endo Agar Plate culture mediums. It is based on the significance value (p-value) showing 0,664 which is higher than the significant level (α = 0,05) (p-value > 0,05). Conclusion: There is no significant difference of Klebsiella pneumoniae colonies number grown on Eosin Methylene Blue and Endo Agar Plate culture mediums

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