Perbedaan Jumlah Koloni Bakteri Escherichia Coli Dengan Suhu Inkubasi 35°C Dan 37°C Pada Media Plate Count Agar

Prasasti, Maria Mahdalena Devin (2019) Perbedaan Jumlah Koloni Bakteri Escherichia Coli Dengan Suhu Inkubasi 35°C Dan 37°C Pada Media Plate Count Agar. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Escherichia coli is a commensal bacterium or normal flora in the peritoneum or lower intestine. Laboratory test is carried out for calculating the colony numbers by incubating the pure colony of Escherichia coli under the incubation temperature of 35oC and 37oC with plate count agar media. This present research aims to determine the differences of colony number of Escherichia coli which is incubated at 35oC and 37oC using Plate Count Agar as the culture media. Method: The research is analytical observation research with cross-sectional design. The research sample is Escherichia coli where the grown media is incubated at Plate count agar media and incubated at 35oC and 37oC. The experiment in each temperature is repeated for 16 times to prove the data is repetable. After 24 hours, the colony numbers are calculated. Result: The average numbers of colonies of Escherichia coli grown on Plate Count Agar Media at the incubation temperatures of 35oC and 37oC are 24 CFU/m Land 26 CFU/mL, respectively, with the significant value of 0.242 which is higher than 0.05 (p-value>0.05). The statistical analysis proved that there is nodifference in the colonies number incubated at 35oC and 37oC on Plate Count Agar Media. Conclusion: The research proves that there is no different in the colonies number incubated at 35oC and 37oC on Plate Count Agar Media.

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