Perbedaan Derajat Aglutinasi Terhadap Variasi Masa Simpan Antisera Pada Suhu 2-8° C Dengan Metode Tabung

Background: Blood grouping requires high-quality antisera to produce accurate
agglutination reactions. Antisera contain specific antibodies that react with
antigens on the surface of erythrocytes. Reagent quality may be affected by storage
temperature and duration.
Objective: To determine differences in agglutination grades in blood grouping
using the tube method with antisera stored for 0, 5, 6, and 7 days at 2–8°C.
Methods: This study used an analytical observational design with a cross-sectional
approach and repeated measurements on the same samples. A total of 30 subjects
were included. Testing was performed using 5% erythrocyte suspensions reacted
with anti-A, anti-B, and anti-AB antisera stored for 0, 5, 6, and 7 days.
Conclusion: Antisera stored at 2–8°C maintained optimal agglutination reactions
for up to seven days in blood grouping using the tube method,agglutination grade
was 4+ for all storage durations. The Kruskal–Wallis test yielded a p-value of 1.000
(p > 0.05), indicating no significant difference in agglutination grades among
antisera stored for 0, 5, 6, and 7 days. .
Recommendation: Antisera stored for up to seven days at 2–8°C may be used for
blood grouping by the tube method, provided that storage conditions and reagent
quality control are properly maintained.