Perbedaan Kadar Asam Urat Menggunakan Tabung Vacutainer Plain dan Tabung Vacutainer Serum Separator dengan Chemistry Analyzer

Background : Uric acid examination is one of the examinations that is often
carried out in clinical laboratories, to detect uric acid levels in the body. The
main uric acid examination material used is a serum sample. The tubes that will
be used for uric acid examination are plain vacutainer tubes and serum separator
vacutainer tubes.
Objective : To determine the difference in uric acid levels, use a vacutainer plain
tubes and vacutainer serum separator tubes using a chemistry analyzer.
Methods : This research is a quantitative research with an analytical
observational research design with a cross sectional approach. The subjects in
this study were 35 students who met the inclusion and exclusion criteria. veins
were collected in plain blood vacutainer tubes and ± 2 mL serum separator
vacutainer tubes which would then be centrifuged to obtain serum which was
examined using a Kenza 450 TX instrument. Statistical tests use the Paired t Test
with a confidence level of 95%.
Results : The average uric acid level using a plain vacutainer tube was 5.33
mg/dL. The average uric acid level using a serum separator vacutainer tube was
5.19 mg/dL. By using the paired t test, p = 0.000 (p < 0.05), which means there is
a difference in uric acid levels using a vacutainer plain tube and a vacutainer
serum separator tube with a chemistry analyzer.
Conclusion : There was a significant difference in uric acid levels using plain
vacutainer tubes and serum separator vacutainer tubes.
Suggestion : For further research using plain vacutainer tubes and SST
vacutainer tubes, it is recommended to use a larger number of samples with a
variety of research subjects that can influence the results, such as patients with a
history of gout, to determine the effect of the gel separator on uric acid levels,
using different methods. Plain vacutainer tubes are recommended as blood
collection tubes for checking uric acid with a chemistry analyzer.