Perbedaan Kadar Glukosa Darah Sewaktu Pada Serum Yang Langsung Diperiksa dan Plasma EDTA Yang Ditunda 2,5 Jam.

Ferdinand, Yokhana (2022) Perbedaan Kadar Glukosa Darah Sewaktu Pada Serum Yang Langsung Diperiksa dan Plasma EDTA Yang Ditunda 2,5 Jam. Undergraduate thesis, Universitas Katolik Musi Charitas Palembang.

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Abstract

Background: The use of serum samples is more effective in checking blood sugar because it does not have coagulation factors that can affect the results of the examination. Plasma contains blood clotting substances so it must be added with anticoagulants which can affect the results of the examination. Objective: To determine differences in glucose levels using serum and plasma samples of EDTA which were delayed for 2.5 hours Methods: This research is an analytic observational study using a cross-sectional approach using a total sampling technique. The research subjects used were 30 students of the Medical Laboratory Technology Study Program. The tool used is the Biosystem BA-200 with the control material Biochemistry Control Serum Lv. 1 and Lv. 2, the method verification test has been carried out first. Results: Blood glucose levels with serum samples for the mean results obtained were 99.24 mg/dL and the distribution of SD data was 17.56. Blood glucose levels in EDTA plasma samples that were delayed 2.5 hours, the mean results obtained were 99.96 mg/dL and the distribution of Sd data was 17.85. There was no significant difference in the results of examination of blood glucose levels with serum and plasma samples of EDTA which were delayed 2.5 hours, with a sig (2-tailed) value of 0.184 > 0.05. Conclusion: Based on the research, it was concluded that there was no significant difference in the results of the examination of blood glucose levels with serum and plasma samples of EDTA which were delayed for 2.5 hours. Suggestion: Further research is recommended to use patients who have diabetes or by using fasting treatment for glucose examination, and further research is needed on samples using other types of anticoagulants, because anticoagulants affect the results of the examination.

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