Perbedaan Kadar Alkaline Phospatase (ALP) Dalam Serum Darah Yang Disentrifugasi 5000 RPM Selama 1 Menit dan 3000 RPM Selama 5 Menit

Saragih, Krisman Andreas (2021) Perbedaan Kadar Alkaline Phospatase (ALP) Dalam Serum Darah Yang Disentrifugasi 5000 RPM Selama 1 Menit dan 3000 RPM Selama 5 Menit. Diploma thesis, Universitas Katolik Musi Charitas.

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Abstract

Alkaline Phospatase (ALP) is an intracellular enzyme that is very important for the body, so the result of the examination must be released quickly. One that can be done is increase the speed of centrifugation so it decreased time for centrifuge. This research conducted centrifugation of serum at 5000 rpm for 1 minute and 3000 rpm for 5 minutes. Objective : To determine the diffrence in serum ALP levels from blood centrifuged at 5000 rpm for 1 minute and 3000 rpm for 5 minute. This type of research is Pre-experimental with a static group comparison research design. The subjects of this study were 32 respondents who had met the inclusion and exclusion criteria. The blood samples were then frozen at room temperature for 20 – 30 minute, then centrifuged at 3000 rpm for 5 minute and 5000 rpm for 1 minute. The examination method used is IFCC. The data were analyzed using Paired t Test statistical test. Alkaline Phospatase (ALP) levels in blood serum that were still within normal limits. The normality test shows that the data is normally distributed, so to test the hypothesis using a Paired T test. The result of the there is no significant difference in serum from blood centrifuged at 5000 rpm for 1 minute and 3000 rpm for 5 minute wiht a p-value (Sig2-tailed) 0f 0,855 > 0,05. Based on the resultsof this study, it can be conclude that there is no diffrence in the level of Alkaline Phospatase (ALP) in serum from blood centrifuged at 5000 rpm for 1 minute and 3000 rpm for 5 minute. Suggestion : Applicatively the management of examination material are the centrifugation speed of 5000 rpm for 1 minute, can be an alternative of consideration in the ALP examination.

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