PERBEDAAN PEMERIKSAAN KADAR KOLESTEROL TOTAL METODE CHOD-PAP (CHOLESTEROL OXIDASE PEROXIDASE AMINOANTIPYRINE) DAN METODE POCT (POINT OF CARE TESTING)

Simbolon, Rosmani (2021) PERBEDAAN PEMERIKSAAN KADAR KOLESTEROL TOTAL METODE CHOD-PAP (CHOLESTEROL OXIDASE PEROXIDASE AMINOANTIPYRINE) DAN METODE POCT (POINT OF CARE TESTING). Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Laboratory is a health service. Clinical chemistry examination is part of laboratory. The clinical chemistry section has many types of examinations, one of them is a total cholesterol test. Examination of Cholesterol levels can be applied with several examination methods. Nouddays using the CHOD-PAP method is the standard reference. The POCT method of Total cholesterol examination is interesting to examine because of due to less precision and accuracy compared to the reference method. Objective: Recognize the difference in the examination total cholesterol levels using serum samples using the CHOD-PAP method and capillary blood samples using the POCT method. Method: Comparative Research method is research that is searching differences or similarities between two or more variables. The research was carried out in Novitiate and the Sister of Charity training center. The research used a cross sectional design. The sampling technique is total sampling. Sampling was carried out according to the inclusion and exclusion criteria. Using 30 samples serum material for examination of the CHOD-PAP method with Biosystem BA200. POCT examination method was carried out from the finger capillary blood spot using Benecheck. Data is analyzed using Shapiro-Wilk test then followed by parametric Independen sample t test Result: The results of examination of total cholesterol levels using the CHOD-PAP method obtained an average of 169.90 mg/dl and the average of total cholesterol using POCT method obtained of 185.74 mg/dl. The of result Independent samples t test obtained a p-value 0.015 (p value (< 0.05). Conclusion: Based on the results of this research, it can be concluded that total cholesterol levels examination using CHOD-PAP method and POCT method has a significant difference. Suggestion: The nest exemination is separated total cholesterol examination using CHOD-PAP method and POCT method using control materials samples from the factory. Keywords: cholesterol levels, CHOD-PAP method, POCT method

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