Perbedaan Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung menggunakan Suspensi Sel Darah Merah yang dilakukan Pencucian Ulang dan Tanpa Pencucian Ulang

Aulia, Intan (2026) Perbedaan Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung menggunakan Suspensi Sel Darah Merah yang dilakukan Pencucian Ulang dan Tanpa Pencucian Ulang. Undergraduate thesis, Universitas Katolik Musi Charitas Palembang.

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Abstract

Background: Blood typing is performed to determine blood group type, with the tube method serving as the gold standard, which includes serum grouping and cell grouping using a 5% erythrocyte suspension. Test results can be influenced by erythrocyte concentration and the red blood cell washing process using a 0.9% NaCl solution. Objective: To determine differences in the degree of agglutination in tube-based blood typing using red blood cell suspensions that were rewashed and those that were not rewashed after 24 hours of storage at 2–8°C. Methods: An analytical observational study with a cross-sectional design. The sample consisted of 5% red blood cell suspensions from 33 students in the DIV Medical Laboratory Technology program. Testing was performed after 24 hours of storage. Data were analyzed using the Wilcoxon signed-rank test. Results: Of the 33 samples with two different treatments, the results using a 5% red blood cell suspension-both with and without rewashing after 24 hours of storage-showed a 4+ agglutination level in all samples. Conclusion: Based on the study results, it was concluded that there was no difference in the degree of agglutination during blood typing using a 5% RBC suspension without rewashing and with rewashing. The results of the Wilcoxon Signed-Rank Test showed a p-value of 1.000 (>0.05). Recommendation: A 5% RBC suspension without rewashing remains suitable for tube blood typing after 24 hours of storage at 2–8°C.

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