Perbedaan Kadar Bilirubin Total Pada Tabung Serum Separator Tanpa Ditutup Aluminium Foil Segera Periksa Dan Ditutup Aluminium Foil Tunda 2 Jam

Sari, Fitri Nur Rohma (2026) Perbedaan Kadar Bilirubin Total Pada Tabung Serum Separator Tanpa Ditutup Aluminium Foil Segera Periksa Dan Ditutup Aluminium Foil Tunda 2 Jam. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Total bilirubin testing is a laboratory test used to assess liver function. Bilirubin is photosensitive and easily changes due to light and temperature exposure. The pre-analytical stage is crucial, as approximately 60–70% of laboratory errors occur at this stage due to improper sample handling. Delays in testing due to limited laboratory equipment and personnel can potentially lead to decreased bilirubin levels in samples and reduce the accuracy of laboratory results. Aluminum foil is used to protect bilirubin samples from light exposure because bilirubin is photosensitive when exposed to light Objective: To determine the difference in total bilirubin levels in serum without aluminum foil (check immediately) and serum covered with aluminum foil (check after 2 hours). Methods: This was a pre-experimental study with a one-group pretest-posttest design. The study was conducted at the Public Health Laboratory Center. Fifty subjects were recruited. Sampling was conducted using a non-probability sampling technique with a purposive sampling method. The data analysis used a Paired Sample T-Test with a 95% confidence level. Results: The average total bilirubin level in serum separator tubes without aluminum foil caps (check immediately) was 0.50 mg/dL, while the average total bilirubin level in serum separator tubes covered with aluminum foil caps was 0.46 mg/dL. Conclusion: There was no difference in total bilirubin levels between serum separator tubes without aluminum foil caps (check immediately) and those covered with aluminum foil caps (delayed 2 hours) with p = 0.110 (<0.05). Recommendation: Further research is recommended to consider other variables such as temperature, sample storage time, and variations in delay time that may affect total bilirubin levels.

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