Perbedaan Diameter Zona Hambat Bakteri Escherichia coli pada Media Mueller Hinton Agar dengan Variasi Jumlah Sterilisasi

Nazara, Giselle Ziona (2025) Perbedaan Diameter Zona Hambat Bakteri Escherichia coli pada Media Mueller Hinton Agar dengan Variasi Jumlah Sterilisasi. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Antibiotic susceptibility testing is used to determine antibiotics effective against bacteria. The Kirby-Bauer diffusion method on Mueller Hinton Agar is widely used. The medium is often prepared in bulk, leaving portions stored for later use. Before reuse, solidified medium is re-liquefied through reheating, usually by autoclaving at 121°C for 15 minutes. Mueller Hinton Agar is known to have good heat resistance, but repeated sterilization may affect its nutrients. Escherichia coli is a common bacterium causing various infections; therefore, antibiotic susceptibility testing is important for its examination.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Inhibition Zone, Escherichia coli, Mueller Hinton Agar, Repeated Sterilization, Autoclave
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Undergraduate Theses - D3/D4 > Medical Laboratory Technology Diploma Study Program
Depositing User: Nazara Giselle Ziona
Date Deposited: 12 Sep 2025 07:27
Last Modified: 12 Sep 2025 07:27
URI: http://eprints.ukmc.ac.id/id/eprint/14729

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