Perbedaan Kadar Bilirubin Total Pada Sampel Serum Yang Segera Diperiksa Dan Ditunda 30 Menit

Ernesta, Agatha Christy (2025) Perbedaan Kadar Bilirubin Total Pada Sampel Serum Yang Segera Diperiksa Dan Ditunda 30 Menit. Undergraduate thesis, Universitas Katolik Musi Charitas.

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Abstract

Background: Total bilirubin testing in clinical laboratories is an important indicator of liver function, but it is highly sensitive to light and temperature. The pre-analytical phase plays a crucial role, as approximately 60–70% of laboratory errors occur at this stage due to improper specimen handling. Delayed testing caused by equipment or personnel limitations may reduce bilirubin levels and affect result accuracy. Objective: To determine the difference in total bilirubin levels between serum samples tested immediately and those tested after a 30-minute delay. Methods: This observational analytic study used a cross-sectional design. A total of 37 subjects aged 18–31 years were selected using a total sampling technique. The study was conducted at the Public Health Laboratory Centre (BBLKM). Data were analysed using a Paired Sample T-Test with a 95% confidence level. Result: The average total bilirubin level in serum samples tested immediately was 0.61 mg/dL, while in those tested after a 30-minute delay it was 0.47 mg/dL. Statistical analysis revealed a significant difference between the two conditions. Conclusion: There is a significant difference in total bilirubin levels between serum samples tested immediately and those tested after a 30-minute delay with p = 0.000 (< 0.05). Suggestion: Future research is recommended to use alternative testing methods and consider other influencing variables such as temperature, light exposure, and storage time, which may affect total bilirubin levels.

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